Gene Expression
Elucidating the Role of Arsenic Toxicity in Hepatocarcinogenesis
A review of Global gene expression associated with hepatocarcinogenesis in adult male mice induced by in utero arsenic exposure.
Note: This is a review of the published article listed below. All information, quotes, figures, methods, and findings mentioned in this review are from that article, and are the property of its authors and/or the publication in which the article originally appeared.
Jie Liu and colleagues (2006) profiled global gene expression associated with hepatocarcinogenesis in adult male mice induced by in utero arsenic exposure. The group used the Agilent mouse oligo 22K microarray to confirm the hepatocarcinogenic potential of arsenic in adult male mice after transplacental exposure, and it also provided an opportunity for a genomewide gene expression analysis. The 22K mouse chip array revealed several novel pathways and gene expression alterations associated with arsenic-induced HCC and in arsenic-exposed nontumorous normal liver samples, which were not detected in previous limited gene expression analysis.
Figure 1. Altered gene expression in adult male mice exposed to arsenic in utero.
The significantly altered genes under criteria of > 1.5-fold difference and p < 0.001 were clustered for comparison. Arsenic-exposed normal liver samples and arsenic-induced liver tumors are compared to control livers. Increased gene expression is shown in red, and decreased gene expression is shown in green.
Figure 2. The Ingenuity Pathways Analysis of gene expression changes.
The network number 1 from arsenic-induced HCC and control liver samples is depicted. The central role of MYC activation in transplacental arsenic carcinogenesis is illustrated. Red indicates increases in gene expression, and green indicates decreases.
Title: Global Gene Expression Associated with Hepatocarcinogenesis in Adult Male Mice Induced by in Utero Arsenic Exposure.
Journal: Environ Health Perspect. 2006 Mar; 114(3): 404-411.
Authors: Liu J, Xie Y, Ducharme DM, Shen J, Diwan BA, Merrick BA, Grissom SF, Tucker CJ, Paules RS, Tennant R, Waalkes MP.
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