Sequencing Libraries for the Illumina Platform
Dr. Michael A. Quail
Head of Sequencing Library Construction
The Wellcome Trust Sanger Institute
Synopsis:
In this presentation, Dr. Quail highlights the work carried out by the Wellcome Trust Sanger Institute, UK in sequencing operations. He outlines the workflow of the sequencing process over the various stages, focusing mainly on Illumina sequencing library preparation. He identifies two main problems in library preparation (adapter dimer formation and contamination from smaller fragments) and suggests methods to overcome these challenges. Using the Agilent Bioanalyzer and the Agilent High Sensitivity DNA Kit,
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