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Agilent’s SureSelect Target Enrichment System significantly improves the cost- and process-efficiency of next-generation sequencing. Based on an extremely efficient hybrid selection technique, the SureSelect Target Enrichment System can be automated and easily scaled to meet the needs of larger sequencing projects. This is unlike any other commercially available target enrichment method. With sample input requirements at or below 3 µg of gDNA, researchers can perform highly targeted next-generation sequencing on the most precious of samples.
The SureSelect Target Enrichment Platform is available through eArray, our online custom microarray design tool. For more information, visit eArray.
Figure 1. SureSelect Target Enrichment System Workflow.
As shown in Figure 1, the SureSelect Target Enrichment System workflow is solution-phase based and is performed in microcentrifuge tubes or microtiter plates. The SureSelect Target Enrichment System was originally designed to work with the Illumina Genome Analyzer end-sequencing protocol but is now also optimized to work with Illumina Genome Analyzer paired-end sequencing and the Applied Biosystems™ SOLiD System, making the SureSelect platform the most universally accepted product for target enrichment commercially available. Utilizing Agilent’s eArray, an intuitive web-based design tool with wizards, researchers can create customized target enrichment kits. Each target enrichment kit comes with the mixture of custom SureSelect RNA oligonucleotides, or baits, that are biotinylated for easy capture onto streptavidin-labeled magnetic beads, as well as buffers and blocking agents necessary for performing the capture process (Figure 1).
Human All Exon Kit now available.
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