Hybridization and Wash
Hybridization and Wash
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Agilent is committed to bringing scientists robust research tools that accelerate and streamline their research. Our microarray processing platform has a long history of being the leader in sensitivity and throughput capabilities. Small sample input requirements, simplified experimental workflows, and easy-to-use protocols all combine to deliver unparalleled handling of genomic samples containing full complexity. Agilent's buffers and reagents contribute to a comprehensive workflow, providing you with the optimized tools you need to obtain quality results. By offering an application-specific workflow, Agilent enhances your ability to conduct microarray experiments, regardless of your experimental needs.
Agilent's hybridization reagents, spike-in kits, and wash buffers enable you to perform reproducible and sensitive microarray analysis using application-specific components, through improved signal-to-noise ratios and higher signal intensities. Actual data confirms that our comprehensive portfolio gives customers the ability to take advantage of the enhanced performance and sensitivity of Agilent's 60-mer oligonucleotide microarrays. It is the first commercial microarray platform compatible with both one- and two-color detection for gene expression, and provides a completely integrated workflow for multiple applications like aCGH and ChIP-on-chip analysis.
Figure 1. Signal intensity comparison plot between experimental replicates.
GeneSpring GX generated plot without flag filters with X-axis representing normalized intensities of 50:50 RNA target (Replicate #1), while the Y-axis represents normalized intensities of 50:50 RNA target (Replicate #2). The two black lines represent the two-fold change cut-off.
Figure 2. Median %CV of the replicated probes per microarray.
The %CV for both replicated non-control probes (in red) and the one-color Spike-In probes (in blue) is depicted. The X-axis represents individual microarray experiments (named after the mixed RNA sample) performed in triplicate.
Figure 3. Dynamic range and linearity of the Spike-In controls.
Data representing the green signal for each Spike-In transcript is plotted against the log of the relative concentration for one microarray. The line shown on the plot represents the linear range based on a parametric curve fit though the data.
Reference: Performance of the Agilent Microarray Platform for One-Color Analysis of Gene Expression.
Agilent Publication Number 5989-4486EN.
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Gene Expression Hybridization Kit: hybridize fluorescently-labeled cRNA to microarrays for more reproducible and consistent results.
For detailed specifications and ordering information visit agilent.com
Gene Expression Hybridization Kit, Large Volume: larger reagent volumes to hybridize fluorescently-labeled cRNA to microarrays, including buffers to fragment cRNA down to optimal-sized targets and minimize nonspecific binding.
For detailed specifications and ordering information visit agilent.com
One-Color RNA Spike-In Kit: in vitro-synthesized, polyadenylated transcripts offering minimal self- or cross-hybridization and a 6-order-of-magnitude dynamic range to monitor a microarray workflow for linearity, sensitivity, and accuracy.
For detailed specifications and ordering information visit agilent.com
Two-Color RNA Spike-In Kit: two spike mixes, each comprising 10 in vitro-synthesized, polyadenylated transcripts in predetermined ratios used to obtain expected versus observed log ratios to monitor a microarray workflow for linearity, sensitivity, and accuracy.
For detailed specifications and ordering information visit agilent.com
Oligo aCGH/ChIP-on-chip Hybridization Kit: hybridization solution and blocking agent that are validated and optimized for use in Agilent's Oligonucleotide Array-Based CGH (aCGH) and ChIP-on-Chip workflows for higher signal-to-noise ratios and signal intensities.
For detailed specifications and ordering information visit agilent.com
Gene Expression Wash Buffer Kit: environmentally friendly solutions that enable efficient post-hybridization processing of large numbers of microarrays.
For detailed specifications and ordering information visit agilent.com
Oligo aCGH/ChIP-on-chip Wash Buffer Kit: validated and optimized buffers for use in Agilent's Oligonucleotide Array-Based CGH (aCGH) and ChIP-on-Chip workflows.
For detailed specifications and ordering information visit agilent.com
Stabilization and Drying Solution: a proprietary solution that acts as a chemical, long-term protectant containing an ozone scavenger dissolved at near-saturation levels in acetonitrile to improve the performance of cyanine dye-labeled microarray slides.
For detailed specifications and ordering information visit agilent.com
For research use only and not for use in diagnostic procedures.