Analysis of Model Organisms
Analysis of Model Organisms
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Note: This is a review of the published article listed below. All information, quotes, figures, methods, and findings mentioned in this review are from that article, and are the property of its authors and/or the publication in which the article originally appeared.
Use of model organism systems to elucidate gene function continues to provide valuable insights into gene activity and regulation. Information obtained with model organisms is often helpful in characterizing homologous processes in the human cells and diseases. Bruckmann and colleagues (2004) used Agilent’s yeast oligonucleotide microarrays containing 10,807 60-mer oligonucleotide probes (representing 6,256 known open reading frames (ORFs) from the S. cerevisiae S288C strain to examine transcriptional regulation by the highly conserved yeast 14-3-3 protein family. Agilent’s microarrays helped demonstrate that 14-3-3 proteins negatively regulate Rtg3-dependent transcription, and positively regulate transcription of genes involved in ergosterol biosynthesis and the stress response.
Figure 1. Steady-state mRNA levels of affected genes
A. Log ratio of mRNA levels in GG3096 relative to CEN-PK113-7D detected by the 10,807 oligonucleotide probes on the microarrays. The total data set has equal amounts of OFRs with decreased and increased mRNA levels. B. Log ratio of mRNA levels in GG3096 relative to CEN-PK113-7D of ORFs classified in module 67. Almost all ORFs in the module have decreased mRNA levels. C. Log ratio of mRNA levels in GG3096 relative to CEN-PK113-7D of ORFs with at least two stress-response elements in their promoter regions. These results indicate that decreased 14-3-3 protein activity does not have a clear effect on the expression of Msn2- and Msn4-regulated genes.
Original Research Paper:
Title: Regulation of transcription by Saccharomyces cerevisiae 14-3-3 proteins.
Authors: Bruckmann A, Steensma H , Teixeira de Mattos M , van Heusden G
Journal: Biochem J. 2004 Sep 15; 382(Pt 3): 867-875.
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