Human and Mouse ChIP-on-chip Microarrays
Human and Mouse ChIP-on-chip Microarrays
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The Agilent platform offers a diverse selection of chromatin immunoprecipitation on a chip (ChIP-on-chip) arrays. They feature ~244,000 targeted 60-mer oligonucleotide probes covering coding and non-coding sequences. Agilent's catalog of ChIP-on-chip offerings contains pre-designed, high-resolution microarrays that utilize two-color labeling for enhanced sensitivity, accuracy, and reproducibility. Agilent provides optimized and validated probe design that delivers the high signal-to-noise ratios that are essential for the success of ChIP-on-chip experiments.
While microarray technology is typically associated with characterizing gene expression patterns, the use of ChIP-on-chip has opened the door to deeper exploration and understanding of gene regulation and signalling networks. ChIP-on-chip, also known as Location Analysis (LA), allows you to obtain insightful and robust profiles of DNA-protein binding across entire genomes. The characterization of these molecular events provides a means to elucidate the precise location on genomic DNA where regulatory proteins are bound. Acquire more complete and reliable information for networks of in vivo gene expression in specific cells, tissues, or entire organisms in only a matter of weeks – not months or years.
Figure 1. Broad genomic coverage
Nucleosome modifications, known to be associated with transcriptional regulation, were profiled across the yeast genome using ChIP-on-chip. Shown is a location analysis done on Chromosome IV. Genomic positions of probe regions are arrayed along the x-axis, with the ratio of enrichment for probes along the y-axis. ORFs are depicted as gray rectangles, and arrows indicate the direction of transcription. Conserved binding sites for transcriptional regulators are depicted as colored boxes. Numbers beneath genes represent transcriptional activity (mRNA/h). Enrichment values are shown from trimethylated histone H3 lysine 4, acetylated histone H3 lysine 14, and histone H3. Peaks within the profile indicate occupied sites.
Figure 2. Comprehensive regulatory networks
Using multiple expression datasets, a model for the core transcriptional regulatory network was constructed by identifying and integrating target genes that encode transcription factors and chromatin regulators. Regulators are represented by blue ovals, gene promoters by red rectangles, and putative downstream target genes by gray rectangles.
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Human CpG Island: gain insights into biological processes such as cancer, development, genomic imprinting, gene silencing, and chromatin stability by using a powerful analysis tool for genome-wide measurements on a 244K array
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Human ENCODE: specifically designed for location analysis of human DNA-binding proteins using 153,000+ probes representing specific ENCODE regions of chromosomes 1–22 on a 244K array
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Human Promoter: similar to the human ENCODE array, the promoter array is designed for location analysis of human DNA-binding proteins but targets approximately 17,000 of the best-defined human transcripts on a 244K array
For detailed specifications and ordering information visit agilent.com
Mouse Promoter: designed for location analysis of mouse DNA-binding proteins, targeting approximately 17,000 of the best-defined mouse transcripts on a 244K array
For detailed specifications and ordering information visit agilent.com