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Note: This is a review of the published article listed below. All information, quotes, figures, methods, and findings mentioned in this review are from that article, and are the property of its authors and/or the publication in which the article originally appeared.
Microarray technology has enabled scientists to investigate plant structure and function from a molecular perspective, identifying key regulatory elements in environmental responses. New information obtained from global gene expression profiling in plants has provided a new dimension on agriculture, offering a unique opportunity to maximize agricultural yield using genetic manipulation and awareness. Fujita et al (2005) used the Agilent Arabidopsis 2 Oligo Microarray to establish the function of the abscisic acid-responsive Element Binding Protein 1 (AREB1) as a transcription activator of novel ABA-responsive element (ABRE)-dependent abscisic acid signaling, enhancing drought stress tolerance in Arabidopsis. Combining differential expression analyses using microarrays and RNA gel blots, with information on stress inducibility and cis promoter elements, the group identified two groups of candidate target genes of AREB1. This study provides greater understanding of important underlying AREB1 mechanisms and functions in a novel ABRE-dependent ABA signaling pathway in drought stress.

Figure 1. Regulatory Model of ABA Signaling by AREB1.
AREB1 is postulated to mainly regulate the expression of stress-responsive genes via ABRE sequences in vegetative tissues. Genes downstream of AREB1 have been mapped and likely enhance drought stress tolerance and increase fundamental cellular activities under stress conditions.
Original Research Paper:
Title: AREB1 Is a Transcription Activator of Novel ABRE-Dependent ABA Signaling That Enhances Drought Stress Tolerance in Arabidopsis.
Authors: Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez MM, Seki M, Hiratsu K, Ohme-Takagi M, Shinozaki K, Yamaguchi-Shinozaki K.
Journal: Plant Cell. 2005 Dec; 17(12): 3470-3488.
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Arabidopsis thaliana (3) investigate plant organ functions, growth stages, and stress response using 40,000+ Arabidopsis genes and transcripts represented covering the entire genome on a 4X44K array
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Magnaporthe grisea 2.0: allows gene expression profiling in both pathogen and host (rice) simultaneously by covering the entire genome on a 4X44K array
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Oryza sativa L. ssp. japonica: simultaneously characterize thousands of rice genes associated with biological functions, growth processes, and biotic and abiotic stress response on a 4X44K array
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